Guide to Human Cell Line Authentication

This document was written to set out the essential steps for authenticating human cell lines.

Download the latest version of the Guide to Human Cell Line Authentication here (updated 9 January 2014).

The Guide focuses on short tandem repeat (STR) profiling, as a widely used and accepted authentication method.  The Guide includes sections on:

  • Preparing starting material for STR profiling
  • Performing STR profiling
  • Points to be aware of when comparing cell line samples
  • Using an STR profile database
  • What to do with misidentified cell lines.

You might like to read more about authenticating cell lines.  See our reference pages for authenticating human cell lines and testing non-human cell lines.