Version 8.0 Release Notes Version 8.0 was completed on 24 November 2016 and released on 1 December 2016. The previous version was v7.2. The move to v8.0 indicates a major edit, with the addition of Cellosaurus accession numbers. Cell lines were reviewed by the ICLAC members with the opportunity to vote on new entries. For more information, see voting reference numbers: 140930, 141107, 150219, 150506, 150529, 160115, 160223, 160506, 160714, 160906, 161031. ----- A. Changes to Preamble The preamble was edited as follows: 1. Sentence added: “Cell lines are added after examination of cell line provenance and authentication testing data. An effort has been made to exclude synonymous cell lines that were legitimately established from the same donor e.g. where one cell line was knowingly derived from a parental cell line, or two cell lines were established separately from the same individual.” 2. Sentence added: “Additional cell line information is available through other databases and resources. ‘Cellosaurus AC’ refers to the unique ID number assigned to cell lines by the Cellosaurus database (http://web.expasy.org/cellosaurus/). Cellosaurus acts as a cell line knowledge resource with links to many other online resources.” 3. Text order rearranged to allow for new additions. ----- B. Changes to Structure and Format All tables were edited as follows: 1. Two columns were added for Cellosaurus identifiers. Columns are titled “Misidentified Cell Line, Cellosaurus AC” and “Contaminating Cell Line, Cellosaurus AC”. Cellosaurus ACs were added by Amos Bairoch. 2. Page orientation was changed to Landscape, to keep all cell line information on one page and the Notes on a second page. The Notes are not distributed as part of the PDF but are included in the Excel version that is sent out on request. 3. ICLAC review numbers were added to the notes. These numbers refer to the voting forms sent out for ICLAC members to review each new cell line entry; review numbers are in YYMMDD format from the date of creation of each voting document. ICLAC review numbers using this format will be referenced in the database from v8.0 onwards. ----- C. Changes to Table 1: No Authentic Stock Known Cell Line Summary of Edit 1. all cell lines: Where cell lines are believed to be misidentified but the parental cell line is unknown, all cell lines were listed in the Contaminating cell line column and “genetically identical” was changed to “synonymous cell lines, donor origin unknown”. 2. 2008/C13*5.25 (C13, OV2008/C13) Added to Table 1 (ref: 161031). This cell line was previously included with OV2008 under the name “OV2008/C13”. It has been separated into two for consistency with other entries where derivatives are listed separately. 3. A172TR3 (U251-TR3) Added to Table 1 (ref: 161031). 4. A2008 Merged with OV2008; they share the same contaminant and are likely to be the same cell line under different names (ref: 161031). 5. Ao38 (BTI-Tnao38) Added to Table 1 (ref: 160223). 6. BLIN-1 (also subclone 1E8) Separated into two, “BLIN-1” and “1E8 (BLIN-1 derivative)”, for consistency with other entries where derivatives are listed separately. 7. CNE-1 Moved from More Data Needed to Table 1 (ref: 150506). 8. CNE-2 Moved from More Data Needed to Table 1 (ref: 150506). 9. D-54 MG Added to Table 1 (ref: 160115). 10. H249 Merged with NCI-H249, which was originally entered as a separate cell line. 11. HAC15 Added to Table 1 (ref: 160714). 12. IPRI-OL-7 Added to Table 1 (ref: 160223). 13. IPRI-OL-11 Added to Table 1 (ref: 160223). 14. KM20 Added to Table 1 (ref: 160506). 15. KM20L2 (KM20 derivative) Added to Table 1 (ref: 160506). 16. LC5 (L-132 derivative) Added to Table 1 (ref: 160506). 17. LC5-HIV (L-132 derivative) Added to Table 1 (ref: 160506). 18. MA-104 Contaminating cell line changed from “Vero?” to “Unknown, possibly Vero” and missing PMID added 19. MA-111 Contaminating cell line changed from “Vero?” to “Unknown, possibly Vero”. 20. MEL-WEI Cell line name changed from “ME-WEI” to “MEL-WEI”. 21. MT-1 Edited cell line name to, “MT-1 [Multiple cell lines with same name, see Cellosaurus]”. 22. MT-3 Added to Table 1 (ref: 160115). 23. NCI/ADR-RES (MCF-7/AdrR) Merged with MCF-7/AdrR (NCI/ADR-RES), which was originally entered as a separate cell line. 24. OCM-3 Added to Table 1 (ref: 150529). 25. OCM-8 Added to Table 1 (ref: 150529). 26. OV2008 Edited cell line name to “OV2008 (A2008)” (ref: 161031). 27. OV-1063 Edited contaminant from “unknown” to DU-145 (ref: 160115). 28. Panc 01.28 Added to Table 1 (ref: 160714). 29. Panc 06.03 Added to Table 1 (ref: 160714). 30. T406 Edited cell line name to, “T406 [Multiple cell lines with same name, see Cellosaurus]” 31. TCO-1 Edited contaminant from TCO-2 to TCS (ref: 160906). 32. WKD Merged with Wong-Kilbourne, which was originally entered as a separate cell line; most references use “Wong-Kilbourne derivative” and “WKD” interchangeably. Cell line name was changed to “Wong-Kilbourne derivative (WKD)”. ----- D. Changes to Table 2: Authentic Stock Known Cell Line Change 1. all cell lines Where cell lines are believed to be misidentified but the parental cell line is unknown, all cell lines were listed in the Contaminating cell line column and “genetically identical” was changed to “synonymous cell lines, donor origin unknown”. 2. Amdur II (AMDURII) Moved from Table 1 to Table 2 (ref: 160906). 3. BG-1 Moved from Table 1 to Table 2 (ref: 141107). 4. COLO-720E “Contaminating cell line” changed to COLO704 – this appears to be the most common contaminant in distribution. 5. KARPAS-45 “Authentic stock location” edited to include ECACC, based on additional STR profiling data. 6. OCI-Ly3 Added to Table 2 (ref: 160506). -----