ICLAC Register of Misidentified Cell Lines Version 13 Release Notes Version 13 (v13) was released on 26 April 2024. The previous version was v12, released on 16 January 2023. Additions and major edits were reviewed by the ICLAC members with the opportunity to vote on changes. Voting ID numbers for v13 are: 230306, 230704 and 240206. ----- A. Changes to Preamble Change 1. The website address for Cellosaurus was changed to https://www.cellosaurus.org/. B. Changes to Table 1: No Authentic Stock Known Cell Line Change 1. 1.1B4 Contaminating cell line changed from “PANC-1 / unknown” to “Hybrid, PANC-1 / unknown”. 2. 1.1E7 Contaminating cell line changed from “PANC-1 / unknown” to “Hybrid, PANC-1 / unknown”. 3. ACCNS Actual species changed from “Mouse” to “Mouse, Mus musculus”. 4. ACN Added to Table 1 (reference: 230306). 5. BCaP-37 Added to Table 1 (reference: 230306). 6. BHP 18-21 Contaminating cell line changed from “M14 / TPC-1” to “Mixture, M14 / TPC-1”; actual cell type changed from “Melanoma / thyroid, papillary carcinoma” to “Mixture, melanoma, papillary thyroid carcinoma”. 7. CAC2 Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 8. CCL3 Actual species changed from “Mouse” to “Mouse, Mus musculus”. 9. CHB Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 10. Clom 15 Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 11. CM-319 Added to Table 1 (reference: 230306). 12. EC9706 Added to Table 1 (reference: 240206). 13. GPS-M Additional reference added and comment updated; actual species changed from “Mouse” to “Mouse, Mus musculus”. 14. GPS-PD Additional reference added; actual species changed from “Mouse” to “Mouse, Mus musculus”. 15. GREF-X Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 16. HBC Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 17. HHUA Added to Table 1 (reference: 230704). 18. HKTN-2 Added to Table 1 (reference: 240206). 19. Hs 677.St Actual species changed from “Mouse” to “Mouse, Mus musculus”. 20. Huker Added to Table 1 (reference: 240206). 21. IPDDC-A2 Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 22. IPRB Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 23. IPTP/98 Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 24. JHPI-1 clone-16 (Clone-16) Alternative name added; actual species changed from “Syrian hamster” to “Golden hamster, Mesocricetus auratus”. 25. KM3 Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 26. LN-464 Added to Table 1 (reference: 230306). 27. LT-1 Claimed species changed to “Northern leopard frog, Lithobates pipiens”; contaminating cell line changed to “Mixture, FHM / TH”; actual species changed to “Mixture, fathead minnow, Pimephales promelas (FHM) / common box turtle, Terrapene carolina (TH)”; actual cell type changed to “Mixture, unspecified (FHM) / heart (TH)”; reference and comment added. 28. McCoy Actual species changed from “Mouse” to “Mouse, Mus musculus (C3H/An strain). 29. MGC-803 (MGc80-3) Alternative name added; contaminating cell line changed from “HeLa” to “Hybrid, HeLa / unknown”; actual cell type changed from “cervical adenocarcinoma” to “hybrid, cervical adenocarcinoma / unknown; new references added (Cao et al, 2024; Yang et al, 2024); comment altered to reflect new data and conclusions from these two references. 30. NCC16 Actual cell type changed from “Skin, immortalised keratinocytes” to “Skin, immortalized keratinocytes”. 31. PMF-ko14 Added to Table 1 (reference: 230704). 32. RBHF-1 Actual species changed from “Non-human” to “Unknown, not human”. 33. REH-6 Actual species changed from “Mouse” to “Mouse, Mus musculus”. 34. SNG-M Added to Table 1 (reference: 230704). 35. UPES/C Actual species changed from “Mixture, human + mouse” to “Mixture, human / mouse”; comment updated for clarity. 36. UPHHJA Actual species changed from “Rat” to “Rat, Rattus norvegicus”. 37. VC312R Actual species changed from “Rat” to “Rat, Rattus norvegicus”. ----- C. Changes to Table 2: Authentic Stock Known Cell Line Change 1. 1205Lu (WM793 derivative) Actual species changed from “Mixture, human + mouse” to “Mixture, human / mouse”. 2. 207 (EU-2) Contaminating cell line changed from “REH and SUP-B2” to “Mixture, REH / SUP-B2”. 3. Eca-109 (EC109) Added to Table 2 (reference: 240206); new reference added (Gu et al, 2022). 4. JHH-1 Actual species changed from “Mouse” to “Mouse, Mus musculus”. 5. Yamada Actual species changed from “Mouse” to “Mouse, Mus musculus”. ----- D. Changes to More Data Needed table (internal use for ICLAC members) Cell Line Change 1. AlAb Added to More Data Needed table (reference: 230306). 2. HCC-56 Added to More Data Needed table (reference: 230704). 3. NCI-H2691 Added to More Data Needed table (reference: 230704). 4. NCI-H2803 Added to More Data Needed table (reference: 230704). -----