Further Reading – Microbial Contamination


Caputo JL (1988) Biosafety procedures in cell culture. J Tissue Culture Methods 11: 223-227.  See Journal Website.

Centers for Disease Control and Prevention (2009) Biosafety in Microbiological and Biomedical Laboratories, 5th edition.  See CDC Website.

Coelho AC, García Díez J (2015) Biological Risks and Laboratory-Acquired Infections: A Reality That Cannot be Ignored in Health Biotechnology. Front Bioeng Biotechnol 3: 56. PMID: 25973418.

Kimman TG, Smit E, Klein MR (2008) Evidence-based biosafety: a review of the principles and effectiveness of microbiological containment measures. Clin Microbiol Rev 21: 403-25. PMID: 18625678.

Mahy BW, Dykewicz C, Fisher-Hoch S, Ostroff S, Tipple M, Sanchez A (1991) Virus zoonoses and their potential for contamination of cell cultures. Dev Biol Stand 75: 183-9. PMID: 1794619.

Mycoplasma Contamination

McGarrity GJ (1976) Spread and control of mycoplasmal infection of cell cultures. In Vitro 12: 643-8. PMID: 1034618.

Razin S, Hayflick L (2010) Highlights of mycoplasma research-An historical perspective. Biologicals 38: 183-90. PMID: 20149687.

Uphoff CC, Drexler HG (2014) Detection of Mycoplasma contamination in cell cultures. Curr Protoc Mol Biol 106: 28.4.1-28.4.14. PMID: 24733240.

Uphoff CC, Drexler HG (2014) Eradication of Mycoplasma contaminations from cell cultures. Curr Protoc Mol Biol 106: 28.5.1-28.5.12. PMID: 24733241.

Volokhov DV, Graham LJ, Brorson KA, Chizhikov VE (2011) Mycoplasma Testing of Cell Substrates and Biologics: Review of Alternative Non-Microbiological Techniques. Mol Cell Probes 25: 69-77. PMID: 21232597.

Young L, Sung J, Stacey G, Masters JR (2010) Detection of Mycoplasma in cell cultures. Nat Protoc 5: 929-34. PMID: 20431538.

Viral Contamination

Cao S, Strong MJ, Wang X, Moss WN, Concha M, Lin Z, O’Grady T, Baddoo M, Fewell C, Renne R, Flemington EK (2014) High-Throughput RNA sequencing based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project. J Virol pii: JVI.02570-14. PMID: 25355872.

Nims RW (2006) Detection of adventitious viruses in biologicals–a rare occurrence. Dev Biol (Basel) 123: 153-64; discussion 183-97. PMID: 16566443.

Uphoff CC, Denkmann SA, Steube KG, Drexler HG (2010) Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in human and other primate cell lines. J Biomed Biotechnol. 2010: 904767. PMID: 20454443.

Uphoff CC, Lange S, Denkmann SA, Garritsen HS, Drexler HG (2015) Prevalence and characterization of murine leukemia virus contamination in human cell lines. PLoS One 10: e0125622. PMID: 25927683.

Zhang YA, Maitra A, Hsieh JT, Rudin CM, Peacock CD, Karikari C, Brekken RA, Stastny V, Gao B, Girard L, Wistuba I, Frenkel E, Minna JD, Gazdar AF (2011) Frequent detection of infectious xenotropic murine leukemia virus (XMLV) in human cultures established from mouse xenografts. Cancer Biol Ther 12: 617-28. PMID: 21750403.

Image Credit: Tom Deerinck, NIGMS, NIH (via NCI Visuals Online). Public domain.