Guide to Human Cell Line Authentication
Download the latest version of the Guide to Human Cell Line Authentication (updated 2 March 2023).
This document was written to set out the essential steps for authenticating human cell lines.
The Guide focuses on short tandem repeat (STR) profiling, as a widely used and accepted authentication method. The Guide includes sections on:
- Preparing starting material for STR profiling
- Generating STR profiles
- Points to be aware of when comparing cell line samples
- Using an STR profile database
- What to do with misidentified cell lines.